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... an ELISA reader (Multiskan Ascent, Labsystem, Vantaa, Finland) at 450 nm. 2.8. Th1-Th17 Phenotipagem Spleens were aseptically removed and splenocytes were ... permeabilized using eBiosciences' intracellular fixation (Thermo Scientific, ... (BD Biosciences) and analyzed with the flow cytometer's proprietary software.. The absorbance was measured using MULTISKAN JX (Thermo Fisher Scientific, ... Ascent Software for Multiskan Ascent (Thermo Fisher Scientific, Worsham, MA, USA). ... Funding: KY (17K10241) received grant for scientific research from the ... a43a48e1fd